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Nucleic Acids Research, 1985, Vol. 13, No. 20 7183-7194
© 1985

Articles

Ozonolysis of supercoiled pBR322 DNA resulting in strand scission to open circular DNA1

Kazuyuki Sawadaishi, Kazunobu Miura, Eiko Ohtsuka, Tohru Ueda, Kazo Ishizaki* and Nariko Shinriki*

Faculty of Pharmaceutical Sciences, Hokkaido University Sapporo 060, Japan *Government Industrial Development Laboratory Hokkaido, Sapporo 061-01, Japan

Received September 10, 1985. Accepted September 27, 1985.

Treatment of supercoiled pBR322 DNA with ozone resulted in the conversion of closed circular DNA to open circular DNA. Restriction analysis of the resulting open circular DNA showed that ozonolysis in the absence of salt caused single strand cleavage at specific sites.


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