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Nucleic Acids Research, 1985, Vol. 13, No. 20 7427-7444
© 1985

Articles

Low ionic strength extraction of nuclease-treated nuclei destroys the attachment of transcriptionally active DNA to the nuclear skeleton

S.V. Razin, O.V. Yarovaya and G.P. Georgiev

Institute of Molecular Biology, USSR Academy of Sciences Vavilov str. 32, 117984 Moscow B-334, USSR

Received May 9, 1985. Revised September 9, 1985. Accepted September 9, 1985.

We have studied how the conditions in which the nuclear matrix is isolated influence the association of transcribing DNA with the nuclear matrix. Extraction of nuclease-treated naclei with a low ionic strength solution before a high salt extraction completely abolishes this association. However, RNA removal by RNAase treatment does not affect the binding of transcribing DNA to the nuclear matrix. The nature of the association of active genes with the unclear matrix is discussed.


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