Nucleic Acids Research, 1985, Vol. 13, No. 20 7473-7481
© 1985
Articles |
Sequence of the T4 recombination gene, uvsX, and its comparison with that of the recA gene of Escherichia coil
Department of Botany, Faculty of Science, Kyoto University Kyoto 606, Japan
Received August 15, 1985. Accepted September 23, 1985.
We have determined the nucleotide sequence of the uvsX gene of bacteriophage T4 which is involved in DNA recombination and damage repair, and whose product catalyzes in vitro reactions related to recombination process in analogous manners to E. coil recA gene product. The coding region consisted of 1170 nucleotides directing the synthesis of a polypeptide of 390 amino acids in length with a calculated molecular weight of 43,760. Amino acid composition, the sequence of seven NH2- terminal amino acids and molecular weight of the protein deduced from the nucleotide sequence were consistent with the data from the analysis of the purified uvsX protein. The nucleotide sequence and the deduced amino acid sequence were campared with those of the recA gene. Although a significant homology was not found in the nucleotide sequences, the amino acid sequences included 23% of identical and 15% of conservatively substituted residues.
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