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Nucleic Acids Research, 1985, Vol. 13, No. 21 7579-7589
© 1985

Articles

Molecular cloning and structure of the human interleukin 2 receptor gene

Norio Ishida, Hiroshi Kanamori, Takafumi Noma, Toshio Nikaido, Hisataka Sabe, Noboru Suzuki, Akira Shimizu and Tasuku Honjo

Department of Medical Chemistry, Kyoto University Faculty of Medicine Sakyo-ku, Kyoto 606, Japan

Received September 9, 1985. Accepted October 15, 1985.

We have cloned the IL-2 receptor gene from human genomic DNA libraries using IL-2 receptor cDNA as probe. The genomic DNA segments that hybridized with cDNA were subcloned in M13 phages and their sequences were determined. The nucleotide sequences showed that the IL-2 receptor gene was encoded by eight exons and that the coding region sequences agreed completely with that of the IL-2 receptor cDNA cloned from a cell line derived from adult T cell leukemia (ATL), in which IL-2 receptors are expressed abnormally. The nucleotide sequence of the 5'-flanking region had a putative promoter region, which had some homology with the human IL-2 gene. Transcription initiation sites were clustered about 25 bp 3' to the TATA box as assessed by primer extension analysis. These sites for normal and ATL T cells were the same. Exons 2 and 4 encoding the extracytoplasmic portion had significant homology, suggesting that the two exons are derived by duplication of an ancestral exon. Exon 2 contained six cysteine residues, four of which are conserved at the corresponding positions in exon 4.


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