Nucleic Acids Research, 1985, Vol. 13, No. 21 7607-7620
© 1985
Articles |
The nucleotide sequence of the nitrogen-regulation gene ntrA of Klebsiella pneumoniae and comparison with conserved features in bacterial RNA polymerase sigma factors
AFRC Unit of Nitrogen Fixation, University of Sussex Brighton BN1 9RQ, UK
Received September 6, 1985. Accepted October 8, 1985.
The nucleotide sequence of the Klebsiella pneumoniae ntrA gene has been determined. NtrA encodes a 53,926 Dalton acidic polypeptide; a calculated molecular weight which is significantly lower than that determined by SDS polyacrylamide gel analysis. NtrA is followed by another open-reading frame (orf) of at least 75 amino acids. In the spacer region between ntrA and orf there are no apparent transcription termination or promoter sequences and therefore orf may be co-transcribed with ntrA.
Previous authors have proposed that NtrA could act as an RNA polymerase sigma factor but the NtrA amino acid sequence does not show a high level of homology to any known sigma factor. However analysis of sequences of five sigma factors from E. coli and B. subtilis has identified two conserved sequences at the C-terminal end of all these polypeptides. These sequences resemble those found in known site-specific DNA-binding domains and may be involved in recognition of conserved -35 and -10 promoter sequences. A similar pair of sequences is present at the C-terminus of NtrA and could play a role in recognition of ntr-activatable promoters.
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  M. Chaney, R. Grande, S. R. Wigneshweraraj, W. Cannon, P. Casaz, M.-T. Gallegos, J. Schumacher, S. Jones, S. Elderkin, A. E. Dago, et al. Binding of transcriptional activators to sigma 54 in the presence of the transition state analog ADP-aluminum fluoride: insights into activator mechanochemical action Genes & Dev., September 1, 2001; 15(17): 2282 - 2294. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Buck, M.-T. Gallegos, D. J. Studholme, Y. Guo, and J. D. Gralla The Bacterial Enhancer-Dependent sigma 54 (sigma N) Transcription Factor J. Bacteriol., August 1, 2000; 182(15): 4129 - 4136. [Full Text]
|
|
|
|
 |
|
 P. Casaz and M. Buck Probing the assembly of transcription initiation complexes through changes in sigma N protease sensitivity PNAS, October 28, 1997; 94(22): 12145 - 12150. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. V. Cannon, M. K. Chaney, X.-Y. Wang, and M. Buck Two domains within sigma N (sigma 54) cooperate for DNA binding PNAS, May 13, 1997; 94(10): 5006 - 5011. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Tintut, J. T. Wang, and J. D. Gralla Abortive Cycling and the Release of Polymerase for Elongation at the [IMAGE]54-dependent glnAp2 Promoter J. Biol. Chem., October 13, 1995; 270(41): 24392 - 24398. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y Tintut, J T Wang, and J D Gralla A novel bacterial transcription cycle involving sigma 54. Genes & Dev., September 15, 1995; 9(18): 2305 - 2313. [Abstract] [PDF]
|
|
|
|
|
|
B. S. Powell, D. L. Court, T. Inada, Y. Nakamura, V. Michotey, X. Cui, A. Reizer, M. H. Saier Jr., and J. Reizer Novel Proteins of the Phosphotransferase System Encoded within the rpoN Operon of Escherichia coli J. Biol. Chem., March 3, 1995; 270(9): 4822 - 4839. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y V Brun and L Shapiro A temporally controlled sigma-factor is required for polar morphogenesis and normal cell division in Caulobacter. Genes & Dev., December 1, 1992; 6(12a): 2395 - 2408. [Abstract] [PDF]
|
|
|
|
 |
|
 D. Popham, D Szeto, J Keener, and S Kustu Function of a bacterial activator protein that binds to transcriptional enhancers Science, February 3, 1989; 243(4891): 629 - 635. [Abstract] [PDF]
|
|
|
|
|
|
M. Chaney, M. Pitt, and M. Buck Sequences within the DNA Cross-linking Patch of sigma 54 Involved in Promoter Recognition, sigma Isomerization, and Open Complex Formation J. Biol. Chem., July 14, 2000; 275(29): 22104 - 22113. [Abstract] [Full Text] [PDF]
|
|