Skip Navigation

This Article
Right arrow Print PDF (1052K)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Schreiber, G.
Right arrow Articles by Neubert, W. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Schreiber, G.
Right arrow Articles by Neubert, W. J.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1985, Vol. 13, No. 21 7663-7672
© 1985

Articles

Selective protection of in vitro synthesized cDNA against nucleases by incorporation of phosphorothioate-analogues

Georg Schreiber, Edith M. Koch and Wolfgang J. Neubert

Max-Planck-Institut für Biochemie, Abteilung für Virusforschung 8033 Martinsried bei München, FRG

Received August 5, 1985. Revised September 26, 1985. Accepted October 15, 1985.

The conditions for the stepwise synthesis of single- (ss) and double-stranded (ds) cDNA using thio-analogues instead of dNTPs are described in this paper. RNA of paramyxovirus Sendai (strain 6/94) aervea as template in these experiments. The increased resistance of this {propto}S-modified cDNA against several nucleases, like Sl-Nuclease, DNase I, Exonuclease III, snake venom Phosphodiesterase (PDE) and the combination of DNase I and PDE is demonstrated.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?




Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.