Nucleic Acids Research, 1985, Vol. 13, No. 21 7715-7728
© 1985
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Location of the serine residue involved in the linkage between the terminal protein and the DNA of phage 
29
Centro de Biología Molecular (CSIC-UAM), Universidad Autónoma, Canto Blanco 28049 Madrid 1Servicio de Endocrinología, Centro Ramón y Cajal Carretera de Colmenar Viejo Km 9,100, 28034 Madrid, Spain
Received July 12, 1985. Revised October 2, 1985. Accepted October 2, 1985.
B. subtilis phage 
29 has a terminal protein, p3, covalently linked to the 5'ends of the DNA through a phosphodiester bond between a serine residue and 5'-dAMP. This protein acts as a primer in DNA replication by forming an initiation complex with the 5'-terminal nucleotide dAMP.
The amino acid sequence of the terminal protein, deduced from the nucleotide sequence of gene 3, showed the presence of 18 serine residues in a total of 266 amino acids. In this paper we have identified the serine involved in the linkage with the DNA as the residue 232, located close to the C-terminus of the molecule. This result was obtained by amino acid analysis of the peptide that remains linked to the DNA after proteinase K digestion of the terminal protein-29 DNA complex and automated Edman degradation of the corresponding {125I}-labeled tryptic peptide. Prediction of the secondary structure of the terminal protein suggested that the serine residue involved in the linkage with the DNA is placed in a ß-turn, probably located on the external part of the molecule, as indicated by hydropathic values.
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