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Nucleic Acids Research, 1985, Vol. 13, No. 21 7899-7908
© 1985

Articles

Comparative nucleotide sequence analysis of two types of larval ß-globin mRNAs of Xenopus laevis

Walter Knöchel, Wolfgang Meyerhof, Jürg Stalder1 and Rudolf Weber1

Institut für Molekularbiologie und Biochemie der Freien Universität Berlin Arnimallee 22, D-1000 Berlin 33, FRG 1Zoologisches Institut der Universität Berlin, Abteilung fü Zell- und Entwicklungsbiologie Baltzerstrasse 4, CH-3012 Bern, Switzerland

Received July 30, 1985. Revised October 1, 1985. Accepted October 1, 1985.

The complete nucleotide sequence of the cDNA insert of the clone pXGL25 derived from the larval ßII-globin mRNA of Xenopus laevis has been determined. The sequence of 593 nucleotides represents part of the 5'nontranslated region, the coding region for 146 amino acids and the entire 3'nontranslated region. It diverges from the related larval ßI-sequence by 24.9% in the coding region. Alignment of the 5' and 3' nontranslated regions of the two related larval ß-sequences to maximum matching resulted in 31.2% and 46.7% divergence, respectively. Divergence between the corresponding adult and larval sequences considerably exceeds that of related larval sequences, suggesting that larval genes may have arisen by gene duplication prior to genome duplication. In contrast to mammalian ß-globin mRNAs, replacement and silent base substitutions are equally abundant, thus indicating less functional constraint on the larval Xenopus laevis ß-globin chains. The larval ßI and ßII-globins diverge by 30.8% and show most variation in the {alpha}1/ß2-chain interaction sites.


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