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Nucleic Acids Research, 1985, Vol. 13, No. 22 7945-7957
© 1985

Articles

Transcription from a plant gene promoter in animal cells

Elizabeth Dennis* and Paul Berg

Department of Biochemistry, Stanford University Stanford, CA 94305, USA

Received October 7, 1985. Accepted October 24, 1985.

The promoter segment of a plant gene (maize alcohol dehydrogenase 1 (Adh1)) has been fused to two bacterial reporter genes, Ecogpt (1) and neo (2), in Psv2-derived vectors and introduced into cultured mammalian cells by DNA transfection. The pAdh1-gpt plasmids transformed the recipient cells for resistance to mycophenolic acid plus xanthine (3) and the analogous pAdh1-neo plasmid transformed cells to G418 resistance (2). S1 analysis of transient transfections of CV1 cells with various derivatives of pAdh1-gpt confirmed that production of gpt mRNA is initiated at the Adh1 promoter at and near the same site used in transcription of the intact Adh1 gene in maize. Moreover, expression of the Adh1 promoter was increased 10–20 fold if the SV40 early region enhancer sequence was included in the same molecule.

*On leave from CSIRO Division of Plant Industry, Canberra ACT 2601, Australia


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