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Nucleic Acids Research, 1985, Vol. 13, No. 23 8305-8321
© 1985

Articles

Detection of a subgenomic mRNA for gene V, the putative reverse transcriptase gene of cauliflower mosaic virus

Aine L. Plant, Simon N. Covey1 and Donald Grierson

Department of Physiology and Environmental Science, University of Nottingham, School of Agriculture Sutton Bonington, Loughborough LE12 5RD 1Department of Virus Research, John Innes Institute Colney Lane, Norwich NR4 7UH, UK

Received October 8, 1985. Revised November 7, 1985. Accepted November 7, 1985.

Polypeptides synthesized in vitro in rabbit reticulocyte lysates, directed by poly(A)+ RNA isolated from turnip leaves infected with cauliflower mosaic virus (CaMV), were analysed by polyacrylamide gel electrophoresis. Following translation of virus-specific RNA purified by hybrid-selection using CaMV DNA immobilized on DBM papers, the polypeptides observed included the viral gene VI inclusion body protein P62, and a larger product, P75, together with several smaller polypeptides. By translating RNA hybrid-selected with restriction fragments encompassing the CaMV genome, a mRNA for P75 has been mapped to gene V. These results, together with sucrose gradient ultra-centrifugation studies, suggest that a CaMV gene V mRNA is a sub-genomic transcript of approximately 2.5Kb and 22S. The expression strategy of the CaMV genome is discussed in the light of our findings.


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