Skip Navigation

This Article
Right arrow Print PDF (2629K)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by LaFlamme, S. E.
Right arrow Articles by Mills, D. R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by LaFlamme, S. E.
Right arrow Articles by Mills, D. R.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1985, Vol. 13, No. 23 8425-8440
© 1985

Articles

Comparison of pausing during transcription and replication

Susan E. LaFlamme, Fred Russell Kramer and Donald R. Mills

Institute of Cancer Research and Department of Human Genetics and Development, College of Physicians and Surgeons, Columbia University New York, NY 10032, USA

Received September 9, 1985. Revised November 1, 1985. Accepted November 1, 1985.

Pausing during the transcription of MDV-1 cDNA by Escherichia coli RNA polymerase was compared with pausing during the replication of MDV-1 RNA by Qß replicase. MDV-1 RNA is able to form many strong hairpin structures, and Qß replicase pauses after the synthesis of each [Mills et a1. (1978) Cell 15, 541–550]. Although the transcripts were virtually identical to MDV-1 RNA, the locations at which RNA polymerase paused were different and apparently were not related to sequences that can form hairpins. These results indicate that hairpin stability, per se, cannot be used to predict the occurrence of pausing during transcription.

Four pauses that occur within a 5-nucleotide region were studied in detail. Insertions and deletions were made in the template DNA to determine the contribution made by the surrounding sequences to these pauses. The results indicate that some of the pauses require the presence of particular upstream sequences, while others are unaffected by the template modifications. Thus, there are at least two different transcriptional pausing mechanisms: one depends on the nature of upstream sequences, while the other is independent of upstream sequences.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 J. Biol. Chem.Home page
I. Artsimovitch, V. Svetlov, K. S. Murakami, and R. Landick
Co-overexpression of Escherichia coli RNA Polymerase Subunits Allows Isolation and Analysis of Mutant Enzymes Lacking Lineage-specific Sequence Insertions
J. Biol. Chem., March 28, 2003; 278(14): 12344 - 12355.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
J. Ederth, I. Artsimovitch, L. A. Isaksson, and R. Landick
The Downstream DNA Jaw of Bacterial RNA Polymerase Facilitates Both Transcriptional Initiation and Pausing
J. Biol. Chem., September 27, 2002; 277(40): 37456 - 37463.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.