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Nucleic Acids Research, 1985, Vol. 13, No. 23 8477-8486
© 1985

Articles

Human tumor cell lines with EGF receptor gene amplification in the absence of aberrant sized mRNAs

C.Richter King, Matthias H. Kraus, Lewis T. Williams+, Glenn T. Merlino*, Ira H. Pastan* and Stuart A. Aaronson

Laboratory of Cellular and Molecular Biology, National Cancer Institute Bethesda, MD 20892 *Laboratory of Molecular Biology, National Cancer Institute Bethesda, MD 20892 +San Francisco Howard Hughes Institute, Medical Institute U-426 San Francisco, CA 94143, USA

Received July 29, 1985. Revised October 24, 1985. Accepted October 24, 1985.

A survey of himan tumor cell lines for increased PDGF or EGF receptors identified 5 lines which bind from 6 to 13 times more EGF than normal human fibroblasts. Immunoprecipitation analysis links the elevated binding activity to increased quantities of the EGF receptor protein. EGF receptor gene amplification was detected in 2 of the cell lines. No evidence for EGF receptor gene rearrangements was found at the level of DNA or RNA structure. The results suggest that elevated levels of EGF receptor can be associated with at least three distinct mechanisms. These include gene amplification accompanied by rearrangement, gene amplification without accompanied alteration of mRNA transcripts, and extensive expression without gene amplification.


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