Nucleic Acids Research, 1985, Vol. 13, No. 23 8543-8559
© 1985
Articles |
Analysis of cloned cDNA and genomic sequences for phaytochrome: complete amino acid sequences for two gene products expressed in etiolated Avena
Department of Botany, University of Wisconsin Madison, Wl 53706 1Agrigenetics Advanced Research Laboratory Madison, WI 53716, USA
Received August 5, 1985. Revised October 25, 1985. Accepted October 29, 1985.
Cloned cDNA and genomic sequences have been analyzed to deduce the amino acid sequence of phytochrome from etiolated Avena. Restriction endonuclease site polymorphism between clones indicates that at least four phytochrome genes are expressed in this tissue. Sequence analysis of two complete and one partial coding region shows 
98% homology at both the nucleotide and amino acid levels, with the majority of amino acid changes being conservative. High sequence homology is also found in the 5'-untranslated region but significant divergence occurs in the 3'-untranslated region. The phytochrome polypeptides are 1128 amino acid residues long corresponding to a molecular mass of 125 kdaltons. The known protein sequence at the chromophore attachment site occurs only once in the polypeptide, establishing that phytochrome has a single chromophore per monomer covalently linked to Cys-321. Computer analyses of the amino acid sequences have provided predictions regarding a number of structural features of the phytochrome molecule.
+Present Address: E.L. du Pont de Nemours & Company, Central Research and evelopment Department, Experimental Station, Wilmington, DE 19898
*Present Address: Plant Breeding Institute, Trumpington, Cambridge, CB2 2LQ England
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