Skip Navigation

This Article
Right arrow Print PDF (486K)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Asano, M.
Right arrow Articles by Kawade, Y.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Asano, M.
Right arrow Articles by Kawade, Y.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1985, Vol. 13, No. 23 8573-8586
© 1985

Articles

SV40 vector with early gene replacement efficient in transducing exogenous DNA into mammalian cells

Masahide Asano, Yoichiro Iwakura and Yoshimi Kawade

Institute for Virus Research, Kyoto University Sakyo-ku, Kyoto 606, Japan

Received August 29, 1985. Revised November 5, 1985. Accepted November 5, 1985.

An early replacement SV40 vector, SV40-Muß, was constructed by replacing part of the early genes of the virus with mouse interferon-ß (IFN-ß) cDNA. Upon transfection of COS-7 cells with this DNA, transducing viral particles were produced, which could infect various cells and cause efficient production of mouse IFN-ß. The viral stock contained no detectable wild-type SV40. The IFN production after the virus infection was very high in monkey kidney cells, but less so in human epithelial cells, and low in mouse, pig, hamster cells and in human lymphocytes. The efficiency of introduction of the DNA to monkey kidney cells was compared with that by the calcium phosphate precipitation method, and the viral vector was found to be more efficient by a factor of several tens to hundreds.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 J. Biol. Chem.Home page
D. S. Strayer and D. S. Strayer
SV40 As an Effective Gene Transfer Vector in Vivo
J. Biol. Chem., October 4, 1996; 271(40): 24741 - 24746.
[Abstract] [Full Text] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.