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Nucleic Acids Research, 1985, Vol. 13, No. 24 8685-8694
© 1985

Articles

AccIII, a new restriction endonuclease from Acinetobacter calcoaceticus

Keiko Kita, Nobutsugu Hiraoka, Atsushi Oshima, Satoko Kadonishi and Akira Obayashi

Central Research Laboratories, Takara Shuzo Co. Ltd. Seta 3-4-1, Otsu-shi, Shiga 520-21, Japan

Received November 6, 1985. Accepted November 26, 1985.

A new site-specific restriction endonuclease, AccIII, was isolated from Acinetobacter calcoaceticus. AccIII recognizes T{downarrow}CCGGA and cleaves at the position shown by the arrow. AccIII activity was inhibited by adenine methylation at the overlapping dam methylase recognition sequence.


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