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Nucleic Acids Research, 1985, Vol. 13, No. 24 8813-8826
© 1985

Articles

Molecular cloning of human apolipoprotein B cDNA

P. Carlsson, S.O. Olofsson, G. Bondjers, C. Darnfors, O. Wiklund and G. Bjursell

Departments of Medical Biochemistry and Medicine I, University of Göteborg P.O. Box 33031, S-400 33 Göteborg, Sweden

Received September 4, 1985. Revised November 21, 1985. Accepted November 21, 1985.

In this paper we describe the isolation of cDNA clones which code for parts of apolipoprotein B (apoB). The clones were obtained by immunoscreening of an expression library ({lambda}gt 11) derived from a human hepatoma cell line (Hep G2). The relationship between positive clones and apoB was established with immunochemical techniques using polyclonal as well as monoclonal antibodies. Recombinants, expressing nonoverlapping regions of apoB are described, all hybridizing with a very large mRNA (approximately 20,000 bases long). The nucleotide sequence obtained predicts a primary protein structure with a composition suitable for the formation of stretches of an amphipatic {alpha}-helix.


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