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Nucleic Acids Research, 1985, Vol. 13, No. 24 9031-9042
© 1985

Articles

Correlation between the rate of productive transcription initiation and the strand-melting property of Escheriehia coli promoters

Hideki Tachibana and Akira Ishihama

Graduate School of Science and Technology, Kobe University Rokko-dai, Nada-ku, Kobe 657, Japan Department of Molecular Genetics, National Institute of Genetics Mishima, Shizuoka 411, Japan

Received March 22, 1985. Revised November 6, 1985. Accepted November 25, 1985.

"Opening potential"s of DNA segments of about ten base pairs in length were calculated for eleven promoters of Escheriehia coli using the thermodynamic parameter values [Gotoh and Tagashira (1981) Biopolymers 20, 1043–1058] for the stabilities of ten kinds of nearest neighbor base pair doublets in a melting reaction. They were compared with the strength of each promoter determined experimentally with a "mixed transcription" system [Kajitani and Ishihama (1983) Nucleic Acids Res. 11, 3873–3888]. A positive correlation was found between the calculated opening potential in the –9 to +3 region (+1 denotes the nucleotide position at which the transcription starts) and the rate of open complex formation.


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