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Nucleic Acids Research, 1985, Vol. 13, No. 3 711-726
© 1985


Articles

Epidermal growth factor or serum stimulation of rat fibroblasts induces an elevation in mRNA levels for lactate dehydrogenase and other glycolytic enzymes

Lynn M. Matrisian, Guy Rautmann, Bruce E. Magun* and Richard Breathnach

Laboratoire de Genétique Moléculaire des Eucaryotes du CNRS, Unité 184 de Biologic Moléculaire et de Génie Génétique de I'INSERM, Institut de Chimie Biologique, Faculté de Médecine 11, rue Humann, 67085 Strasbourg Cédex, France

Received December 4, 1984. Revised January 14, 1985. Accepted January 14, 1985.

We have isolated cloned cDNAs corresponding to five mRNAs whose level is increased following stimulation of quiescent rat fibroblasts by either epidermal growth factor or serum. Partial sequencing followed by a computer search of data banks has shown that the cloned cDNA5 correspond to mRNAs encoding proteins with extensive homology to lactate dehydrogenase, glyceraldehyde 3-phosphate dehydrogenase, enol ase, tn ose phosphate isomerase, and actin. The complete nucleotide sequence of a rat fibroblast lactate dehydrogenase is presented.


*on sabbatical leave from the University of Arizona. Present address: Dept. Cell Biol. and Anatomy, Oregon Health Sciences Univ., 3181 Sam Jackson Rd, Portland, Oregon, 91201, USA.


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