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Nucleic Acids Research, 1985, Vol. 13, No. 3 791-804
© 1985

Articles

Organization, structure and expression of murine interferon alpha genes

Ellen C. Zwarthoff, Arno T A. Mooren and Jan Trapman

Department of Pathology, Erasmus University P.O. Box 1738, 3000 DR Rotterdam, The Netherlands

Received November 12, 1984. Accepted January 9, 1985.

Using a human interferon-{alpha} probe we have isolated recombinant phages containing murine interferon-alpha (Mu IFN-{alpha}) genes from a genomic library. One of these phages contained two complete Mu IFN-{alpha} genes and part of a third gene. The insert of a second phage held two IFN genes. This indicates that the Mu IFN-{alpha} genes are clustered in the genome as is the case for the analogous human genes. The nucleotide sequences of these 5 genes were determined. They show that the genes are all different, albeit highly homologous. The deduced amino acid sequences show that four of the five genes contain a putative glycosylation site. Three genes were transiently expressed in COS cells and they gave rise to protein products showing ant iviral properties.

The expression of the five Mu IFN-{alpha} genes and the Mu IFN-ß gene was studied in virus-induced mouse L cells. The individual mRNAs were visualized in a nuclease S1 experiment, using a specific probe for each gene. In RNA preparations from induced cells mRNAs for each of the five a genes and the 13 gene were present. However, substantial differences in the amounts of the individual mRNAs were observed.


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