Skip Navigation

This Article
Right arrow Print PDF (5811K)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (38)
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Thompson, R. J.
Right arrow Articles by Mosig, G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Thompson, R. J.
Right arrow Articles by Mosig, G.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1985, Vol. 13, No. 3 873-891
© 1985

Articles

An ATP-dependent supercoiling topoisomerase of Chlamydomonas reinhardtii affects accumulation of specific chforoplast transcripts

Robert John Thompson and Gisela Mosig

Department of Molecular Biology, Vanderbilt University Nashville, TN 37235, USA

Received July 13, 1984. Revised November 21, 1984. Accepted December 31, 1984.

We have found that Chlamydomonas reinhardtii cells contain an ATP- dependent topoisomerase activity that supercoils circular DNA in vitro. Subsequent addition of a type I topoisomerase eliminates the supercoils. Like bacterial gyrase, this activity is inhibited by low concentrations of novobiocin (<0.1 µM) and by nalidixic acid (<0.1 mM). We have examined the effects of these topoisomerase inhibitors on accumulation of various chloroplast transcripts in vivo. Novobiocin differentially affected such transcripts; some transcripts became more abundant while many others were reduced in the presence of this drug. Nalidixic acid on the other hand caused many transcripts to become more abundant albeit to varying degrees.

Inhibitors of this algal topoisomerase specifically stimulate a family of related transcripts which we have previously shown to be under light-dark control. We discuss how the inhibitors of this topoisomerase might exert their in vivo effects.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 GeneticsHome page
N. G. Nossal, J. L. Franklin, E. Kutter, and J. W. Drake
Gisela Mosig
Genetics, November 1, 2004; 168(3): 1097 - 1104.
[Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
M. K. Wall, L. A. Mitchenall, and A. Maxwell
Arabidopsis thaliana DNA gyrase is targeted to chloroplasts and mitochondria
PNAS, May 18, 2004; 101(20): 7821 - 7826.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
D. S. Lupold, A. G. F. S. Caoile, and D. B. Stern
Genomic context influences the activity of maize mitochondrial cox2 promoters
PNAS, September 28, 1999; 96(20): 11670 - 11675.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
M. L. Salvador, U. Klein, and L. Bogorad
Endogenous Fluctuations of DNA Topology in the Chloroplast of Chlamydomonas reinhardtii
Mol. Cell. Biol., December 1, 1998; 18(12): 7235 - 7242.
[Abstract] [Full Text]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.