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Nucleic Acids Research, 1985, Vol. 13, No. 3 893-909
© 1985

Articles

Translationally coupled initiation of protein synthesis in Bacillus subtilis

Rolf Sprengel*, Bernd Reiss+ and Heinz Schaller

Department of Microbiology, University of Heidelberg Im Neuenheimer Feld 230, Heidelberg, FRG

Received December 19, 1984. Accepted January 16, 1985.

The neomycin phosphotransferase gene (neo) from Transposon Tn5 is active in Gram-negative bacteria but silent in B.subtilis since it lacks an appropriate ribosome binding site for Gram-positive bacteria. Neo translation could be reactivated by coupling its initiation to the translational termination of the highly expressed ß-lactamase gene ( from B.licheniformis. This initiation occured at the authentic neo start codon. Its efficiency was independent of the nucleotide sequence 5' to the neo gene, but strongly affected by the distance between the termination and initiation codon. It was the highest if both codons overlapped in the sequence ATGA. In B.licheniformis, a translationally coupled neo gene was inducible expressed as the penP gene demonstrating the potential of the technique to monitor the activity of expression units for which no direct assays exists.

*Present address: Department of Microbiology and Immunology, University of California, San Francisco, CA 94143, USA

+Present address: Department of Biochemistry, Biological Sciences West, University of Arizona, Tucson, AZ 85721, USA


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