Nucleic Acids Research, 1985, Vol. 13, No. 4 1057-1072
© 1985
Articles |
Drosophila topoisomerase II double-strand DNA cleavage: analysis of DNA sequence homology at the cleavage site
Duke University Medical Center, Department of Biochemistiy Durham, NC 27710, USA
Received December 27, 1984. Accepted January 29, 1985.
In order to study the sequence specificity of double-strand DNA cleavage by Drosophila topoisomerase II, we have mapped and sequenced 16 strong and 47 weak cleavage sites in the recombinant plasmid p
25.1. Analysis of the nucleotide and dinucleotide frequencies in the region near the site of phosphodiester bond breakage revealed a nonrandom distribution. The nucleotide frequencies observed would occur by chance with a probability less than 0.05. The consensus sequence we derived is 5'GT.A/TAY
ATT.AT ..G 3', where a dot means no preferred nucleotide, Y is for pyrimidine, and the arrow shows the point of bond cleavage. On average, strong sites match the consensus better than weak sites.
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