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Nucleic Acids Research, 1985, Vol. 13, No. 4 1193-1208
© 1985

Articles

The extent of DNA sequence required for a functional bacterial attachment site of phage lambda

Michiyo Mizuuchi and Kiyoshi Mizuuchi

Laboratory of Molecular Biology, National Institute of Arthritis, Diabetes, and Digestive and Kidney Diseases, National Institutes of Health Bethesda, MD 20205, USA

Received November 30, 1984. Accepted January 23, 1985.

We have investigated the extent of DNA sequence required to form a bacterial attachment site (attB) that functions in bacteriophage {gamma} Integration. A DNA fragment carrying attB of Escherlchia coli was trimmed, recloned and tested for recombination proficiency. We found that the common core sequence plus the adjoining 4-bp sequences of both the B and B' arms are required for full activity, while plasmids with an even shorter attB eequence retain some capacity to function as attB in vivo. We also found that the nonspecific DNA that is joined to the required attachment site sequence does not significantly influence the rate of the recombination reaction.


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