Skip Navigation

This Article
Right arrow Print PDF (2167K)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (64)
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Chang, K. S.
Right arrow Articles by Schwartz, R. J
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Chang, K. S.
Right arrow Articles by Schwartz, R. J
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1985, Vol. 13, No. 4 1223-1237
© 1985

Articles

The complete sequence of the chicken {alpha}-cardiac actin gene: a highly conserved vertebrate gene

Kun Sang Chang, Katrina N. Rothblum and Robert J Schwartz*

Department of Cell Biology, Baylor College of Medicine Houston, TX 77030, USA

*To whom correspondence should be addressed

Received November 23, 1984. Revised January 29, 1985. Accepted January 29, 1985.

We sequenced the entire chicken {alpha}-cardiac actin gene. A single intron was positioned 20 bp upstream from the initiation ATG codon in the 5' non-coding region while the coding region was interrupted by 5 introns at amino acid positions 41/42, 150, 204, 267, and 327/328.Sequencing allowed the first comparison of the {alpha}-cardiac and {alpha}-skeletal actin transcriptional promoters. These highly G+C rich promoters share two regions of homology which are found at position –134 (10 bp) end –296 (12 bp) in the {alpha}-cardiac actin promoter. A smaller 9 bp motif (CCGCCCCGG) homologous to the –134 sequence was detected before, between and after the TATA and CAAT boxes of the {alpha}-cardiac actin gene. The polyadenylation signal (AATAAA) was located 156 bp downstream from the translation termination codon. The complete length of the {alpha}-cardiac actin mRNA excluding the poly A tail is 1370 nucleotides. The 3' noncoding transcribed portion of the chicken {alpha}-cardiac actin gene was found to be extraordinarily conserved when compared to the human and rat {alpha}-cardiac actin mRNA sequences.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Mol. Cell. Biol.Home page
A. A. Hill and P. R. Riley
Differential Regulation of Hand1 Homodimer and Hand1-E12 Heterodimer Activity by the Cofactor FHL2
Mol. Cell. Biol., November 15, 2004; 24(22): 9835 - 9847.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
C. Y. Chen and R. J. Schwartz
Identification of Novel DNA Binding Targets and Regulatory Domains of a Murine Tinman Homeodomain Factor, nkx-2.5
J. Biol. Chem., June 30, 1995; 270(26): 15628 - 15633.
[Abstract] [Full Text] [PDF]

Home page
 ScienceHome page
S. Berman, S Bursztajn, B Bowen, and W Gilbert
Localization of an acetylcholine receptor intron to the nuclear membrane
Science, January 12, 1990; 247(4939): 212 - 214.
[Abstract] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.