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Nucleic Acids Research, 1985, Vol. 13, No. 4 1251-1266
© 1985

Articles

rDNA in Locusta, migratoria is very variable: two introns and extensive restriction site polymorphisms in the spacer

Mireille Schafer and Werner Kunz

Institut für Genetik, Universität Düsseldorf, Universitatsstr 1, D-4000 Düsseldorf, FRG

Received November 20, 1984. Revised January 21, 1985. Accepted January 21, 1985.

Cloned ribosonal DNA (rDNA) of Locusta migratoria was analyzed by restriction site mapping and SI nuclease experiments. The repeat unit is 18 kb long. The nontranscribed spacer region (NTS) is very large (11 kb) and homogeneous in length, but many of the restriction sites are heterogeneous among the repeat units. Two introns of different length were found at different positions in the 28S gene. They are present in less than 5% of the genes.


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