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Nucleic Acids Research, 1985, Vol. 13, No. 4 1317-1326
© 1985

Articles

Construction of a vector pRSVcatamb38 for the rapid and sensitive assay of amber suppression in human and other mammalian cells

Julian F. Burke* and Anne E. Mogg

MRC Cell Mutation Unit, University of Sussex Falmer, Brighton, Sussex BN1 9RR, UK

Received December 31, 1984. Revised January 25, 1985. Accepted January 25, 1985.

We describe the generation of an amber mutation in the chloramphenicol acetyltransferase (cat) gene of the mammalian cell transfection vector pRSVcat (Gorman et.al. (1982), Proc.Natl.Acad.Sci. 79 6777–6791). We have demonstrated the in vivo suppression of this amber mutation in monkey and human cells by cotransfection with a synthetic Xenopus suppressor tRNATyr under the control of the late SV40 promoter. The vector, pRSVcatamb38, may be used to quantitate amber suppression in various mammalian cells.

*Present address: Department of Biochemistry, School of Biological Sciences, University of Sussex, Falmer, Brighton, Sussex BN1 9QG, UK


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