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Nucleic Acids Research, 1985, Vol. 13, No. 5 1483-1492
© 1985

Articles

Stimulation of the UvrABC enzyme-catalyzed repair reactions by the UvrD protein (DNA helicase II)

Keiko Kumura, Mutsuo Sekiguchi, Anne-Lill Stemum* and Erling Seeberg*

Department of Biology, Faculty of Science, Kyushu University Fukuoka 812, Japan *Norwegian Defence Research Establishment, Division for Environmental Toxicology N-2007 Kjeller, Norway

Received January 3, 1985. Accepted February 12, 1985.

An in vitro assay system was constructed using highly purified preparations of UvrA, UvrB, UvrC, UvrD proteins and DNA polymerase I, the objective being to analyse the role of UvrD protein in excision repair of UV-induced DNA damage. UvrABC enzyme-initiated repair synthesis was greatly enhanced by the addition of UvrD protein to the reaction mixture. Further analysis revealed that UvrD protein stimulated introduction of strand breaks in irradiated DNA by UvrABC enzyme but had no effect on the DNA polymerase I reaction. Thus, the site of action of UvrD protein is probably at the incision-excision step and not in later steps in excision repair.


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