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Nucleic Acids Research, 1985, Vol. 13, No. 5 1529-1541
© 1985

Articles

Biotin-labeled synthetic oligodeoxyribonucleotides: chemical synthesis and uses as hybridization probes

André Chollet and Eric H. Kawashima

Department of Chemistry, BIOGEN S.A., 3 route de Troinex CH-1227 Carouge/Geneva, Switzerland

Received December 31, 1984. Revised February 15, 1985. Accepted February 15, 1985.

Oligodeoxynucleotides have been selectively labeled with biotin at their 5'-termini through an aminoalkylphosphoramide linker arm by an efficient chemical method. The reactions were performed in aqueous solution on unprotected oligonucleotides and were insensitive of the sequence and length of the oligonucleotide.

5'-biotin-labeled oligonucleotides were hybridized to dot, Southern and genomic blots of target plasmid DNA immiobilized on nitrocellulose filters. Detection level is about 2 fmole. There is no noticeable disturbance of the strength and selectivity of hybridization of the 5'-biotin-labeled probes in comparison with non-modified DNA.


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