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Nucleic Acids Research, 1985, Vol. 13, No. 5 1593-1606
© 1985

Articles

Nucleotide sequence of a chicken {delta}-crystallin gene

Mutsuhito Ohno, Hiroshi Sakamoto, Kunio Yasuda, T.S. Okala and Yoshiro Shimura*

Department of Biophysics, Faculty of Science, Kyoto University Kyoto 606, Japan

*To whom correspondence should be addressed

Received January 14, 1984. Revised February 1, 1985. Accepted February 1, 1985.

We have determined the complete nucleotide sequence of one of the two non-allelic {delta}-crystallin genes in the chicken, arbitrarily designated {delta}-gene 1, using a genomic clone ({lambda}g{delta}106) containing the entire gene sequence. By comparison of the genomic sequence and the {delta}-crystallin cDNA sequence previously determined, we have identified exon sequences in the genomic sequence. Thus, the presence of 17 exons and 16 introns in the gene has been clarified. The {delta}-crystallin polypeptide deduced from the exon sequences consists of 465 amino acids which is larger, by 19 amino acid residues, than the polypeptide deduced from the cDNA sequence previously reported. Re-examination of the cDNA sequence using the same cDNA clone previously used shows that the present exon sequences are correct and the molecular weight of the deduced {delta}-crystallin polypeptide is 50,615 daltons instead of the previously reported value of 48,447 daltons. In addition, some structural features of the {delta}-crystallin gene including putative expression signals are discussed.


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