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Nucleic Acids Research, 1985, Vol. 13, No. 5 1659-1663
© 1985

Articles

The nucleotide sequences of a cytoplasmic and a chloroplast tRNATyr from Scenedesmus obliquus

Gaynor A. Green* and David S. Jones+

Department of Biochemistry, University of Liverpool P.O. Box 147, Liverpool L69 3BX, UK

+To whom correspondence should be addressed

Received December 17, 1984. Revised February 12, 1985. Accepted February 12, 1985.

The nucleotide sequences of two species of tyrosine accepting tRNA from the eukaryotic green alga Scenedesmus obliquus have been determined. The sequence of the cytoplamic tRNATyr is: pC-C-C-C-U-U-G-U-A-G-C-U-C-A-G-D-D-Gm-G-C-A-G-A-G-C-mFormulaG-G-G-A-G-A-C-U-G-{psi}-A-m1G-A-{psi}-C-U-C-U-A-G-m7Formula-C-A-C-U-G-G-T-{psi}-C-G-m1A-A-U-C-C-G-G-U-C-G-A-G-G-G-G-A-C-C-A-OH and that of the chloroplast tRNATyr is: pG-G-G-C-C-G-A-U-G-C-C-G-A-A-G-U-Formula-Gm-G-D-U-A-A-U-G-G-G-G-C-G-G-A-{psi}-U-G-U-A-(i6 A/ms2i6 A)-A-{psi}-C-C-G-C-U-G-G-U-U-A-C-G-C-C-U-A-C-G-U-U-G-G-T-{psi}-C-G-A-A-U-C-C-G-A-C-U-C-G-G-C-C-C-A-C-C-A-OH. This is the first chloroplast tRNATyr species to be sequenced.

*Present address: Institut de Biologie Moleculaire et Cellulaire du CNRS, Universite Louis Pasteur, 15 rue Rene Descartes, F-67084 Strasbourg, France.


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