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Nucleic Acids Research, 1985, Vol. 13, No. 5 1763-1776
© 1985

Articles

Multiple polyadenylation sites in a Drosophila tropomyasin gene are used to generate functional mRNAs

Mark Boardman1, Guriqbal S. Basi and Robert V. Storti*

Department of Biological Chemistry, University of Illinois Health Science Center Chicago, IL 60612, USA

Received October 29, 1984. Revised February 5, 1985. Accepted February 5, 1985.

The gene encoding muscle tropomyosin I in Drosophila is alternatively spliced in embryonic and thoracic muscle to generate two size classes of RNAs. By Northern blot analysis, the embryonic RNA class shows a broad RNA band of hybridization of 1.3 kb and a more sharply defined, less abundant RNA band at 1.6 kb. The thoracic class of RNAs, on the other hand, consists of a broad hybridization band at 1.7 kb and a more sharply defined band at 1.9 kb. Each size class of RNA encodes a different tropomyosin isoform. The two classes of alternatively spliced RNAs utilize the same 3' terminal exon of the gene. The DNA sequence of this exon reveals a cluster of several polyadenylatlon signals (AAUAAA) or polyadenylation-like signals. We show here by S1 nuclease protection analysis that at least five and possibly seven of these polyadenylatlon or polyadenylation-like sequences are associated with in vivo embryonic and thoracic mRNA cleavage processing sites. Six of these S1 sites are clustered within 119 bp and a seventh is located 255 bp downstream. At least one of the polyadenylation-like signal sequences appears to be an unusual AACAAA sequence. In addition we also show that these mRNAs function in vitro to synthesize muscle tropomyosins.

1Present address: Department of Biological Sciences, University of Warwick, Coventry CV4 7AL, UK

*To whom reprint requests should be sent


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