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Nucleic Acids Research, 1985, Vol. 13, No. 6 1829-1840
© 1985

Articles

Analysis of regulatory sequence upstream of the E. coli uvrB gene; involvement of the DnaA protein

E.A van den Berg1, R.H. Geerse, J. Memelink, R.A.L. Bovenberg, F.A. Magnée and P. van de Putte

Laboratory of Molecular Genetics, University of Leiden Wassenaarseweg 64, 2333 AL Leiden, The Netherlands

*To whom reprint requests should be sent

Received February 4, 1985. Accepted February 28, 1985.

A region located upstream of the uvrB promoters P1 and P2 was found to cause high plasmid loss when cloned in multicopy vectors. Two sequence elements responsible for this phenomenon were identified by mapping of spontaneous mutations that restore plasmid maintenance: a sequence known to have in vitro promoter activity and a partially overlapping sequence that shows extensive homology to recognition sites for the DnaA protein. Accordingly alterations in the level of DnaA protein in vivo were found to affect the extent of plasmid loss. A possible role for interaction of the DnaA protein with the region of interest is discussed in relation to regulation of uvrB expression.

1Present address: Laboratory of Genetics, Free University of Amsterdam, De Boelelaan 1087, 1007 MC Amsterdam, The Netherlands


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