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Nucleic Acids Research, 1985, Vol. 13, No. 6 1841-1853
© 1985

Articles

Structure of the galactokinase gene of Escherichia coli, the last (?) gene of the gal operon

C. Debouck*,+, A. Riccio1,*, D. Schumperli2,*, K. McKenney3,*, J. Jeffers4,+, C. Hughes5,+, M. Rosenberg*,+, M. Heusterspreute, F. Brunel and J. Davison

*Laboratory of Biochemistry, National Cancer Institute, National Institutes of Health Bethesda, MD 20205 +Smith Kline and French Laboratories Philadelphia, PA 19101, USA International Institute of Cellular and Molecular Pathology 75 Avenue Hippocrate, 1200 Brussels, Belgium

Received January 30, 1984. Accepted February 28, 1985.

We present the nucleotide sequence of the galactokinase gene (galK) of Escherichia coli including its 5' and 3' flanking regions. This DNA sequence derives from the {lambda}ga18 transducing phage and is identical to the sequence present in the galk gene fusion vectors, pKO and pKG, commonly used to study transcriptional regulatory elements. We define the precise 3' junction between the bacterial and phage sequences in {lambda}ga18 and demonstrate that this junction prohably results from a homologous recombination event between identical 9 bp sequences common to the gal operon and phage {lambda}. Moreover, we examine the 300 bp region located immediately beyond galK for transcription termination function and find no gal operon terminator. Lastly, we compare the galK genes of E. coli and the yeast S. cerevisiae and find several regions of strong homology among which is a potential ATP-binding site homology shared by a variety of ATP-binding proteins including protein kinases encoded by mammalian oncogenes.

1Present address: Istituto di Patologia Generale II, Facolta Di Medicina, Naples, Italy

2Present address: Institut fur Molekularbiologie II, Universitat Zurich, Winterthurerstrasse 266A, CH-8057 Zurich, Switzerland

3Present address: Laboratory of Molecular Genetics, NINCDS, NIH, Bethesda, MD 20205, USA

4Present address: Ouachita Baptist University, Department of Chemistry, OBU Box 748, Arkadelphia, AR 71923, USA

5Present address: University of Cambridge, Department of Pathology, Cambridge, CB2 1QP, UK


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