Nucleic Acids Research, 1985, Vol. 13, No. 6 1905-1922
© 1985
Articles |
Removal of repeated sequences from hybridisation probes
MRC Mammalian Genome Unit King's Buildings, West Mains Road, Edinburgh, EH9 3JT, UK
Received December 21, 1984. Revised February 22, 1985. Accepted February 22, 1985.
Pre-reassociation of human clone probes, containing dispersed highly repeated sequences, (e.g. Alu and KpnI families), with a large excess of sonicated total human DNA allows signal from single and low copy number components to be detected in transfer hybridisations. The signal from non-dispersed repeated sequences is reduced to single copy levels. The procedure, which is simple and quick, is illustrated using model combinations of well characterised cloned probes, and is applied to a sample of randomly chosen cosmid clones. A theoretical assessment is presented which may be useful to those wishing to use this procedure.
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