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Nucleic Acids Research, 1985, Vol. 13, No. 6 1923-1938
© 1985

Articles

Optimizing the expression in E. coli of a synthetic gene encoding somatomedin-C (IGF-I)

Gary Buell, Marie-Francoise Schulz, Gerald Selzer, Andre Chollet, N.Rao Movva, Dominique Semon, Sonia Escancz and Eric Kawashima

Departments of Molecular Biology and Chesmstiy BIOGEN SA, 3 Route de Troinex, 1227 Carouge/Geneva, Switzerland

Received December 21, 1984. Accepted March 5, 1985.

Double-stranded DNA encoding the human hormone somatomedin-C (SMC) has been synthesized. This synthetic gene hae been inserted into a plasmid bearing the strong leftward promoter (PL) of bacteriophage {lambda} and expressed in E. coli. Codons for the N-terminal region of SMC which maximized the hormone's synthesis were chosen in an SMC-lac z fusion assay. The amounts of SMC accumulated in E. coli were influenced by mutations at two chromosomal loci, lon and htpR.


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