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Nucleic Acids Research, 1985, Vol. 13, No. 6 2063-2074
© 1985


Articles

Cloning and nucleotide sequence of the aspartase gene of Escherichia coli W

Jun S. Takagi, Nobuo Ida, Masanobu Tokushige, Hiroshi Sakamoto* and Yoshiro Shimura*

Department of a Chemistry, Kyoto University Kyoto 606, Japan *Department of Biophysics, Faculty of Science, Kyoto University Kyoto 606, Japan

Received February 1, 1985. Accepted February 22, 1985.

The aspA gene of Escherichia coli W which encodes aspartase was cloned into the plasmid vector pBR322. The nucleotide sequences of aspA and its flanking regions were determined. The aspA gene encodes a protein with a molecular weight of 52,224 consisted of 477 amino acid residues. The amino acid sequence of the protein predicted from the nucleotide sequence was consistent with those of the NH2- and COOH-regions and also with the amino acid composition of the purified aspartase determined previously. Potential promoter and terminator sequences for aspA were also found in the determined sequence.


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