Nucleic Acids Research, 1985, Vol. 13, No. 7 2251-2265
© 1985
Articles |
Structure and function of the region of the replication origin of the Bacillus subtilis chromosome. III. Nucleotide sequence of some 10,000 base pairs in the origin region
Cancer Research Institute, Kanazawa University 13-1, Takaramachi, Kanazawa 920, Japan
*To whom correspondence should be addressed
Received January 12, 1985. Revised March 15, 1985. Accepted March 15, 1985.
Approximately 10,000 nucleotides were sequenced in the oriC region of the Bacillue subtilis chromosome. The first replicating DNA strands are hybridized with a SalI-EcoRI fragment (nucleotide #1206–2954) in one direction (left to right) and an EcoRI-PetI fragment (#2949–4233) in the other. Seven open reading frames (ORF) accompanied with Shine-Dalgarno(SD) sequences were identified. 0RF638 and 0RF821 were identified as gyrB and gyrA genes respectively based on genetic evidences and amino acid sequence data. Comparison of amino acid sequences revealed that 0RF44, 0RF446, 0RF378 and 0RF323 are homologous with rpmH, dnaA, dnaN and recF of Escherichia coli, respectively. Thus, the organization of the ORFs from 0RF44 to 0RF638 resembles the organization of genes in the rpmH-gyrB region of the E. coli chromosome. Two non-coding regions characteristic for oriC signals were found near the site of initiation of the first replicating DNA. They are composed of repeating sequences whose consensus sequence TTAT(C/A)CACA is identical to that of 4 repeating sequences in the oriC of E. coli.
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