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Nucleic Acids Research, 1985, Vol. 13, No. 7 2293-2304
© 1985

Articles

Spacer sequences downstream of the 28S RNA coding region are part of the mouse rDNA transcription unit

Ingrid Grummt, Haralabos Sorbaz*, Ansgar Hofmann and Erika Roth

Institut für Biochemie Röntgenring 11, 8700 Würzburg, FRG *Max-Planck-Institut für Biochemie 8033 Martinsried, FRG

Received January 4, 1985. Revised March 7, 1985. Accepted March 7, 1985.

Evidence is presented that more than 300 bp of spacer sequences downstream of the 28S RNA coding sequence are part of the mouse rDNA transcription unit. Studies in two cell-free transcription systems as well as analysis of cellular RNA indicate that RNA polymerase I does not terminate within the 334 bp 3' terminal spacer sequences contained in the rDNA clone used. Quantitative hybridization data, S1 mapping experiments and Northern analysis of nuclear RNA showed that the 14 kb pre-rRNA molecules hybridize with the same efficiency to both the 28S and the 3' NTS specific DNA probe. This indicates that the rRNA precursor contains both at the 5' and 3' end several hundreds bases of external transcribed spacer sequences which are eliminated in subsequent processing reactions.


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