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Nucleic Acids Research, 1985, Vol. 13, No. 7 2325-2335
© 1985

Articles

Protein - RNA interaction in the rat liver 5S rRNA-protein L51 complex studied by digestion with ribonucleases

B. Gross, H. Welfle* and H. Bielka

Central Institute of Molecular Biology, Department of Cell Physiology, Academy of Sciences of GDR 1115 Berlin-Buch, GDR *Department of Optical Spectroscopy, Academy of Sciences of GDR 1115 Berlin-Buch, GDR

Received December 13, 1984. Revised March 5, 1985. Accepted March 5, 1985.

Protein-RNA interactions in the 5S rRNA-protein L5 complex from rat liver ribosomes were studied by limited digestion of free and protein bound 5S rRNA with ribonuclease A and T1. In the complex with protein L5 the digestion of 5S rRNA by ribonuclease T1 is decreased at G37 and G89, whereas U38 and C39, and to a lower extent also C10 ana U12 become accessible for ritionuclease A.


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