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Nucleic Acids Research, 1985, Vol. 13, No. 7 2519-2531
© 1985

Articles

Analysis of the promoters for the two immunity genes present in the ColE3-CA38 plasmid using two new promoter probe vectors

Kin-F. Chak and Richard James

School of Biological Sciences, University of East Anglia Norwich NR4 7TJ, Norfolk, UK

Received January 3, 1985. Revised March 4, 1985. Accepted March 11, 1985.

We have constructed two new promoter probe vectors which carry a polylinker derived from plasmid pUC19 proximal to the 5' end of a promoter-less galactokinase gene. Using these two vectors we have demonstrated that the ColE3imm gene and the ColE8imm gene present on the ColE3-CA38 plasmid have their own promoters, independent of the SOS promoter of the colicin E3 structural gene. The activity of two terminators, one located proximal to the 5' end of the ColE8imm gene, the other located proximal to the 5' end of the lys gene, were shown by a comparison of the galactokinase activity conferred by several of the recombinant plasmids.


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