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Nucleic Acids Research, 1985, Vol. 13, No. 8 2773-2788
© 1985

Articles

Cloning and nucleotide sequence of the tzs gene from Agrobacterium tumefaciens strain T37

D.E. Akiyoshi*, D.A. Regier, G. Jen1 and M.P. Gordon

Department of Biochemistry, SJ-70, University of Washington Seattle, WA 98195 1Department of Biology, Washington University St. Louis, MO 63130, USA

*To whom correspondence should be addressed

Received January 23, 1985. Revised March 28, 1985. Accepted March 28, 1985.

The trans-zeatin secretion locus (tzs), from the nopaline Ti plasmid of Agrobacterium tumefaciens strain T37, was cloned and the nucleotide sequence determined. This gene is located in the virulence region of pTiT37. The tzs gene is responsible for the secretion of trans-zeatin into bacterial culture medium and in addition has the cytokinin biosynthetic activity, dimethyl-allylpyrophosphate:AMP dimethylallyltransferase. Sequence analysis showed an open reading frame of 729 nucleotides, capable of encoding a protein of 27,545 daltons. A single new labelled protein of 27,200 daltons was detected in Escherichia coli maxicells expressing the cloned tzs gene. Significant sequence homology was observed between the tzs and the published tmr sequence from pTiT37.


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