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Nucleic Acids Research, 1985, Vol. 13, No. 8 2869-2879
© 1985

Articles

Preferential DNase I sensitivity of insert-free ribosomal RNA repeats of Drosophila melanogaster

Richard L. Wayne, Z.Dave Sharp* and James D. Procunier

Biology Department, Rice University Houston, TX 77005, USA

Received November 7, 1984. Revised March 15, 1985. Accepted March 15, 1985.

The five predominant types of rDNA repeats in D. melanogaster were analyzed with respect to their DNase I sensitivity. Only the insert-free repeats showed a generalized DNase I sensitivity pattern whereas the major type I, both minor type I and type II repeats were not as extensively degraded by the nuclease. For XX and XY embryonic nuclei, where there is rapid cell division, the majority of the In– repeats were DNase I sensitive. This indicated that these In– repeats have the potential to be transcribed during this developmental stage. When compared to the In– repeats, the chromatin configuration of the In+ repeats is indicative of a higher order of chromatin folding. The paucity of In+ primary gene transcripts observed in vivo could result from In+ repeats being packaged into a more condensed form of chromatin.

*Present address: Department of Cellular and Structural Biology, University of Texas Health Science Center, San Antonio, TX, USA


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