Skip Navigation

This Article
Right arrow Print PDF (2074K)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Anderson, M.L.M.
Right arrow Articles by Young, B.D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Anderson, M.L.M.
Right arrow Articles by Young, B.D.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1985, Vol. 13, No. 8 2931-2941
© 1985

Articles

Cloning and sequence analysis of an Ig {lambda} light chain mRNA expressed in the Burkitt's lymphoma cell line EB4

M.L.M. Anderson, L. Brown, E. McKenzie, J.E. Kellow and B.D. Young*

Beatson Institute for Cancer Research, Garscube Estate Switchback Road, Bearsden, Glasgow, UK

Received February 18, 1985. Accepted March 22, 1985.

A cDNA library was constructed from the mRNA of the Ig{lambda} producing Burkitt's lymphoma cell line, EB4. Overlapping clones encompassing the coding sequence of the Ig{lambda} mRNA were isolated and sequenced. The predicted amino acid sequence shows a short hydrophobic leader peptide and a mature polypeptide of 217 residues in which V, J and C regions can be distinguished. The V region belongs to subgroup VI and has greatest homology (80%) with the Amyloid-AR protein. The constant region is the Kern– Oz+ isotype. Probing normal human DNA with the subcloned V{lambda} coding sequence detects one gene at high stringency and a family of 11 members at low stringency. To date, no restriction enzyme site polymorphisms have been detected. The V{lambda}VI gene is rearranged on both chromosomes of EB4 and is deleted on both chromosomes in the Burkitt's lymphoma cell line BL2.

*Present address: Imperial Cancer Research Fund, Lincoln's Inn Fields, London


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?




Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.