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Nucleic Acids Research, 1985, Vol. 13, No. 9 3043-3062
© 1985

Articles

Cloning and charactesization of theDAS gene encoding the major methanol assimilatory enzyme from the methylotrophic yeast Hansenula polymorpha

Zbigniew A. Janowicz, Michael R. Eckart, Christel Drewke, Rainer O. Roggenkamp, Cornelis P. Hollenberg, Jan Maat, Aat M. Ledeboer, Chris Visser and C.Theo Verrips

Institut für Mikrobiologie, Universität Düsseldorf 4000 Düsseldorf, FRG Unilever Research Laboratorium Vlaardingen P.O. Box 114, 3130 AC Vlaardingen, The Netherlands

Received March 4, 1985. Accepted April 10, 1985.

A gene library from the methanol utilizing yeast Hansenula polymorpha constructed in a {lambda} Charon4A vector, was used to clone the gene encoding a key methanol assimilating enzyme, dihydroxyacetone synthase (DHAS) by differential plaque hybridization. The nucleotide sequence of the 2106 bp structural gene and the 5' and 3' non-coding regions was determined. The deduced amino acid sequence of the protein is in agreement with the apparent molecular weight and amino acid composition of the purified protein. The codon bias is not so pronounced as in some Saccharomyces cerevisiae genes.


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