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Nucleic Acids Research, 1985, Vol. 13, No. 9 3213-3220
© 1985

Articles

Structure and transcription of the tRNA1Pro gene from Escherichia coli

Yoshiyuki Kuchino, Fumiko Mori and Susumu Nishimura

Biology Division, Nationa Cancer Center Research Institute 5-1-1, Tsukiji, Chuo-ku, Tokyo 104, Japan

Received February 5, 1985. Revised April 10, 1985. Accepted April 10, 1985.

A 5 kbp DNA fragment containing the Formula gene from Escherichia coli was cloned into Charon 21A phage and sequenced by the M13 DNA sequencing technique. When the cloned DNA fragment was used as a template for in vitro transcription with E. coli RNA polymersse, a Formula precursor of 120 nucleotide residues was obtained. The Formula gene transcribed as a single transcription unit was followed by two unusual repeating sequences, both of 108 bp. These two repeating sequences were separated by a 60 bp spacer sequence. The 5'-portion of each repeating sequence overlapped 19 bp of the 3'-terminal region of the Formula gene just like the repeating sequences in the E. coli Formula gene. A rho-independent termination was present in the first repeating unit.


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