Skip Navigation

This Article
Right arrow Print PDF (1978K)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (23)
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Delort, A.-M.
Right arrow Articles by Dinh, S.T.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Delort, A.-M.
Right arrow Articles by Dinh, S.T.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1985, Vol. 13, No. 9 3343-3355
© 1985

Articles

Influence of uracil defect on DNA structure: 1H NMR invastigation 500 MHz

A.-M. Delort*, J.M. Neumann+, D. Molko, M. Hervé+, R. Téoule and S.Tran Dinh+

Laboratoires de Chimie, Département de Recherche Fondamentale, Centre d'Etudes Nucléaires de Grenoble 85 X, F.38041 Grenoble Cédex, France +Service cit Biophysique, Département de Biologie, Centre d'Etudes Nucléaires de Saclay F.91191 Gif-sur-Yvette Cédex, France

*To whom correspondence should be addressed

Received February 25, 1985. Accepted March 22, 1985.

The local structure of two self complementary oligonucleotides d(GTAC-GTAC) and d(GTACGUAC) which differ only by the presence of uracil, not a normal component of DNA, have been investigated by 1H NMR at 500 MHz The two octamera exhibit the same thermodynamical constants (t1/2 {Delta}H), their exchangeable protons broaden and disappear at the same temperature. The T-U substitution did not induce any significant changes on non exchangeable protons re sonances from 2-D COSY and 2-D NOESY experiments. So the two octamers exhibit the same global structure. The only variation was detected by 1D NOE measurements : the base orientations around the N glycosidic bonds (x angles) are different.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?




Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.