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Nucleic Acids Research, 1986, Vol. 14, No. 1 335-349
© 1986

Articles

A computer program package for restriction map analysis and manipulation

Guenther Zehetner and Hans Lehrach

European Molecular Biology Laboratory Meyerhofstrasse 1, D-6900 Heidelberg, FRG

Received June 6, 1985. Programs for the calculation, storage and analysis of restriction maps derived from the analysis of partial digestion products from end labelled DNA (1,2,3) and their correlation with digestion-and hybridisation patterns in total digestions and Southern blot experiments are described. These programs allow direct input of gel patterns from partial or complete digestion experiments using a digitizer tablet, calculation of molecular weights and restriction maps, plotting of maps and actual or predicted fragment patterns and automated identification of overlapping cosmids from partial restriction mapping results. Programs are written in PASCAL and have been implemented on a VAX/VMS system, with a HP-7221T plotter and a digitizing tablet.


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