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Nucleic Acids Research, 1986, Vol. 14, No. 11 4393-4400
© 1986

Articles

The nudeotide sequence of the gene for {gamma}-glutamylcysteine synthetase of Escherichia coli

Kunihiko Watanabe*, Yoshinori Yamano, Kousaku Murata and Akira Kimura

Research Institute for Food Science, Kyoto University Uji, Kyoto 611, Japan

*To whom correspondence should be addressed

Received May 1, 1986. Accepted May 15, 1986.

The nudeotide sequence of the gsh I gene for {gamma}- glutamylcysteine synthetase(GSH I) of Escherichia coli B has been determined. The gsh I structural gene contains 1557 bases in length and predicted a ploypeptide of 518 amino acids with a calculated molecular weight of 58,251. The value is in good agreement with that obtained from gel filtration and SDS/PAGE of GSH I. The initiation codon 5 bp downstream of putative Shine- Dalgarno sequence was an unusual TTG, which encoded methionine. For transcription, two sets of consensus promoter signals(–10 and –35 regions) overlapping each other were identified. The terminator signal shows the favored stem-loop structure with an adequate free energy {bigtriangleup}G = –22.80 kcal/mol.


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