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Nucleic Acids Research, 1986, Vol. 14, No. 11 4421-4436
© 1986

Articles

Uudermethylation of interferon-{gamma} gene in human T cell lines and normal T lymphocytes

Rikiro Fukunaga, Masako Matsuyama*, Hamki Okamura+, Kumiko Nagata+, Shigekazu Nagata§ and Yoshihiro Sokawa

Institute for Virus Research, Kyoto University Sakyo-ku, Kyoto 606 *Public Health Research Institute of Kobe City Chuo-ku, Kobe 650 +Hyogo College of Medicine Nishinomiya, Hyogo 663 §Institute of Medical Science, University of Tokyo Minato-ku, Tokyo 108, Japan

Received April 3, 1986. Accepted May 2, 1986.

The relative levels of DNA methylation at CCGG sequences within and around the interferon-{gamma} (IFN-{gamma}) gene in normal human tissues and cell lines were examined by Southern blot analysis using isoschizomeric restriction enzymes, HpaII and MspI. On the test of normal tissues, the IFN-{gamma} gene was under-methylated only in a small population of T lymphocyte, whereas the gene was fully methylated in T cell-depleted lymphocytes and uterus cells. In TCL-Fuj cell line which is a T cell line producing a high level of IFN-{gamma} spontaneously, the IFN-{gamma} gene was undermethylated. Moreover, the extent of DNA methylation was inversely correlated to the level of expression of the IFN-{gamma} gene in several T cell lines including sublines derived from TCL-Fuj cells. However, partial or complete unmethylation at the CCGG sites of IFN-{gamma} gene was observed in a promyelocytic leukemia cell line and two epithelial cell lines that fail to produce IFN-{gamma} irrespective of induction. These results suggest that undermethy-lation of IFN-{gamma} gene is necessary but not sufficient for its efficient expression.


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