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Nucleic Acids Research, 1986, Vol. 14, No. 11 4437-4451
© 1986

Articles

Complete nucleotide sequence of the Escherichia coti recC gene and of the thyA-recC intergenk region

Paul W. Finch, Rosemary E. Wilson, Kate Brown, Ian D. Hickson1, Alan E. Tomkinson* and Peter T. Emmerson

1Departments of Biochemistry and Clinical Oncology, The University Newcastle upon Tyne NE1 4LP, UK

Received March 26, 1986. Revised May 12, 1986. Accepted May 12, 1986.

The nuoleotide sequenoe of a 6,000 bp region of the E. coli ohromosome that inoludea the 3' end of the coding region for the thyA gene and the entire recC gene has been determined. The proposed coding region for the RecC protein is 3369 nucleotides long, which would encode a polypeptide consisting of 1122 ami no acids with a calculated moleoular mass of 129 kDa. Hung bean nuolease mapping of a recC specific transcript produced in vivo indioates that transcription of recC is initiated 80 bp upstream of the trans lational start point. A weak promoter sequenoe situated 5' to the transcription initiation point has been identified. In the 1953 bp thyA-recC intergenic region there are three open reading frames that would code for polypeptides of moleoular mass 30 kDa, 13.5 kDa and 12 kDa, respectively. Although the first and third of these open reading frames are preoeded by possible ribosome binding sites, no obvious promoter sequences oould be identified. Moreover, transorlpts for these reading frames could not be detected.

*Present address: Department of Biochemistry, University of California, Berkeley, CA 94720, USA


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